| Frequently Asked Questions |
Live Chat and Frequently Asked Questions are available for this Product. |
| Analysis Note |
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K. |
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The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations. |
| Biochem/physiol Actions |
Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B. |
| Other Notes |
View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer. |
| Preparation Note |
Activated with bovine brain thromboplastin. |
| Physical form |
Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0 |
