I5502 | CAS:367-93-1 | IPTG | Isopropyl β-D-1-thiogalactopyranoside | 异丙基-beta-D-硫代半乳糖吡喃糖苷

I5502    Isopropyl β-D-1-thiogalactopyranoside Sigma-Aldrich  ≥99% (TLC) 窗体底端 货号 号 目录价 RMB I5502-1G 375.57 I5502-5G 1,179.36 I5502-10G 2,259.27 Synonym: IPTG, Isopropyl β-D-thiogalactoside CAS Number: 367-93-1 Empirical Formula (Hill Notation): C9H18O5S Molecular Weight: 238.30 Beilstein Registry Number: 4631 EC Number: 206-703-0 MDL number: MFCD00063273 PubChem Substance ID: 24896069 Description Application IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ. Other Notes Non-metabolizable galactose analog. Reconstitution A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM. 性质 description non-ionic assay ≥99% (TLC) total impurities ≤0.5 mol/mol Dioxane EQP level Premium storage temp. 2-8°C 安全性 Hazard Codes Xn Risk Statements 19-40-66 Safety Statements 36/37 WGK Germany 3